Therapeutic use of autologous hematopoietic bone marrow transplant combined with concentrated platelet in infected surgical wounds: single-center prospective study



Research Article                

American Journal of BioMedicine                  

Volume 12, Issue 2, 2024, Pages 52-58    10.18081/2333-5106/2024.12/52

Nasser Ghaly Yousif  *, Bhat B 2, Hani G. Yousif 3, Guda EN 2

Received 22 December 2023; revised 02 April 2024; accepted 15 April 2024; published 02 May 2024


Abstract

The healing wound is the consecutive process of maintaining tissue integrity including inflammatory, proliferative, and remodeling processes. Stem cells can self-renew, and also to differentiate into all cell types, and are involved in physiological regeneration, use of mesenchymal stem cells deriving from the bone marrow with PRP has potentiated the healing process. We conducted a single-arm pilot prospective analytical study was performed on 15 patients whose mean age was 31 years (range: 29–51 years), with refractory abdominal wounds at a private hospital in Iraq from March 30, 2022, to March 2023. Ten days after the stem cell transplant the re-enforcement with PRP subcutaneous injection around wounds. Small papules of granulation tissue appeared over the wound, and tissue granulation increased from 80% (30.26–80.00%) to 100% (81–100%). At the same time, the wound size was reduced by 60% (52.10–78.23%). The follow-up period was 7–24 months, during which no recurrence of the wound was found. In conclusion, the current study is the first to characterize the safety and efficacy of bone marrow-derived stem cells in combination with PRP in the treatment of refractory abdominal wound infection. All patients achieved a 100% healing rate in a short time, and no treatment-related adverse reactions occurred during the treatment.

Keywords: Bone marrow; Stem cells; PRP; Wound infections 

Copyright © 2024 Yousif, et al. This article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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1. Schultz GS, Chin GA, Moldawer L, Diegelmann RF. Principles of Wound Healing. Diabet. Foot Probl. 2011;77:395-402.
https://doi.org/10.1017/UPO9781922064004.024
2. Swart JF, et al. Haematopoietic stem cell transplantation for autoimmune diseases. Nat Rev Rheumatol. 2017;13(4):244-56.
https://doi.org/10.1038/nrrheum.2017.7
3. Abbaszadeh H, et al. Regenerative potential of Wharton's jelly-derived mesenchymal stem cells: a new horizon of stem cell therapy. J Cell Physiol. 2020;235(12):9230-40.
https://doi.org/10.1002/jcp.29810
4. Chimutengwende-Gordon M, Khan WS. Advances in the use of stem cells and tissue engineering applications in bone repair. Curr Stem Cell Res Ther. 2012;7(2):122-6.
https://doi.org/10.2174/157488812799219036
5. Yu Y, et al. Human embryonic stem cell-derived cardiomyocyte therapy in mouse permanent ischemia and ischemia-reperfusion models. Stem Cell Res Ther. 2019;10(1):167.
https://doi.org/10.1186/s13287-019-1271-4
6. Xie T, Spradling AC. A niche maintaining germ line stem cells in the Drosophila ovary. Science. 2000;290:328-330.
https://doi.org/10.1126/science.290.5490.328
7. Saldaña L, et al. Immunoregulatory potential of mesenchymal stem cells following activation by macrophage-derived soluble factors. Stem Cell Res Ther. 2019;10(1):58.
https://doi.org/10.1186/s13287-019-1156-6
8. hi R, et al. Localization of human adipose-derived stem cells and their effect in repair of diabetic foot ulcers in rats. Stem Cell Res Ther. 2016;7(1):155.
https://doi.org/10.1186/s13287-016-0412-2
9. Marfia G, et al. Mesenchymal stem cells: potential for therapy and treatment of chronic non-healing skin wounds. Organogenesis. 2015;11(4):183-206.
https://doi.org/10.1080/15476278.2015.1126018
10. Hu MS, et al. Mesenchymal Stromal Cells and Cutaneous Wound Healing: A Comprehensive Review of the Background, Role, and Therapeutic Potential. Stem Cells Int. 2018;2018:6901983.
https://doi.org/10.1155/2018/6901983
11. Abdollahi H, Harris LJ, Zhang P, et al. The role of hypoxia in stem cell differentiation and therapeutics. J. Surg. Res. 2011;165:112-117.
https://doi.org/10.1016/j.jss.2009.09.057
12. Hsu YC, Li L, Fuchs E. Emerging interactions between skin stem cells and their niches. Nat Med. 2014;20(8):847-56.
https://doi.org/10.1038/nm.3643
13. Brennan MÁ, Layrolle P, Mooney DJ. Biomaterials functionalized with MSC secreted extracellular vesicles and soluble factors for tissue regeneration. Adv. Funct. Mater. 2020;30:1909125.
https://doi.org/10.1002/adfm.201909125
14. Branski LK, Gauglitz GG, Herndon DN, Jeschke MG. A review of gene and stem cell therapy in cutaneous wound healing. Burns 2009; 35:171-80.
https://doi.org/10.1016/j.burns.2008.03.009
15. Atiyeh BS, Ioannovich J, Al-Amm CA, El-Musa KA. Management of acute and chronic open wounds: the importance of moist environment in optimal wound healing. Curr Pharm Biotechnol. 2002;3:179-95.
https://doi.org/10.2174/1389201023378283
16. Chance TC, Rathbone CR, Kamucheka RM, et al. The effects of cell type and culture condition on the procoagulant activity of human mesenchymal stromal cell-derived extracellular vesicles. J. Trauma Acute Care Surg. 2019;87:S74-S82.
https://doi.org/10.1097/TA.0000000000002225
17. Hernigou P, et al. Subchondral bone or intra-articular injection of bone marrow concentrate mesenchymal stem cells in bilateral knee osteoarthritis: what better postpone knee arthroplasty at fifteen years? A randomized study. Int Orthop. 2021; 45(2):391-9.
https://doi.org/10.1007/s00264-020-04687-7
18. Boulton AJ, Vileikyte L, Ragnarson-Tennvall G, Apelqvist J. The global burden of diabetic foot disease. Lancet 2005; 366:1719-24.
https://doi.org/10.1016/S0140-6736(05)67698-2
19. Maxson S, Lopez EA, Yoo D, et al. Concise review: role of mesenchymal stem cells in wound repair. Stem Cells Transl Med. 2012;1:142-9.
https://doi.org/10.5966/sctm.2011-0018
20. Dabral S, Muecke C, Valasarajan C, et al. A RASSF1A-HIF1α loop drives Warburg effect in cancer and pulmonary hypertension. Nat. Commun. 2019;10:2130.
https://doi.org/10.1038/s41467-019-10044-z
21. Chahal J, et al. Bone marrow mesenchymal stromal cell treatment in patients with osteoarthritis results in overall improvement in pain and symptoms and reduces synovial inflammation. Stem Cells Transl Med. 2019;8(8):746-57.
https://doi.org/10.1002/sctm.18-0183
22. Gibran NS, Heimbach DM. Current status of burn wound pathophysiology. Clin Plast Surg. 2000;27:11-22.
https://doi.org/10.1016/S0094-1298(20)32680-8
23. Silva I, Almeida J, Vasconcelos C. A PRISMA-driven systematic review for predictive risk factors of digital ulcers in systemic sclerosis patients. Autoimmun Rev. 2015;14:140-52.
https://doi.org/10.1016/j.autrev.2014.10.009
24. Eberly AR, Elvert JL, Schuetz AN. Best practices for the pre-analytic phase of anaerobic bacteriology. Clin. Microbiol. Newsl. 2022;44, 63-71.
https://doi.org/10.1016/j.clinmicnews.2022.04.001
25. Ko SH. Nauta A. Wong V. Glotzbach J. Gurtner GC. Longaker MT. The role of stem cells in cutaneous wound healing: what do we really know? Plast Reconstruct Surg. 2011;127(Suppl 1):10S.
https://doi.org/10.1097/PRS.0b013e3181fbe2d8
26. Stappenbeck TS. Miyoshi H. The role of stromal stem cells in tissue regeneration and wound repair. Science. 2009;324:1666.
https://doi.org/10.1126/science.1172687
27. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143.
https://doi.org/10.1126/science.284.5411.143
28. Lee EY, Xia Y, Kim WS, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regene. 2009;17:540.
https://doi.org/10.1111/j.1524-475X.2009.00499.x
29. Maggini J. Mirkin G. Bognanni I, et al. Mouse bone marrow-derived mesenchymal stromal cells turn activated macrophages into a regulatory-like profile. PLoS One. 2010;5:e9252.
https://doi.org/10.1371/journal.pone.0009252
30. Mohyeldin A. Garzon-Muvdi T. Quinones-Hinojosa A. Oxygen in stem cell biology: a critical component of the stem cell niche. Cell Stem Cell. 2010;7:150.
https://doi.org/10.1016/j.stem.2010.07.007
31. Falanga V, Iwamoto S, Chartier M, et al. Autologous bone marrow-derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue Eng. 2007;13:1299.
https://doi.org/10.1089/ten.2006.0278

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Yousif NG, Bhat B, Yousif HG, Guda EN. Therapeutic use of autologous hematopoietic bone marrow transplant combined with concentrated platelet in infected surgical wounds: single-center prospective study. American Journal of BioMedicine 2024; 12(2):52-58.

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APA
Yousif NG, Bhat B, Yousif HG, Guda EN.(2024, May 02).Therapeutic use of autologous hematopoietic bone marrow transplant combined with concentrated platelet in infected surgical wounds: single-center prospective study. Retrieved May 02, 2024, from Ajbm.net website: https://ajbm.net/research-article_122620html/
MLA
Nasser Ghaly Yousif, Bhat B, Hani G. Yousif, Guda EN “Therapeutic use of autologous hematopoietic bone marrow transplant combined with concentrated platelet in infected surgical wounds: single-center prospective study.” Ajbm.net, 02 May. 2024, https://ajbm.net/research-article_122620html/.
HARVARD
Nasser Ghaly Yousif, Bhat B, Hani G. Yousif, Guda EN. (2024) Therapeutic use of autologous hematopoietic bone marrow transplant combined with concentrated platelet in infected surgical wounds: single-center prospective study, Ajbm.net. Available at: https://ajbm.net/research-article_122620html/ (Accessed: May 102, 2024).
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Nasser Ghaly Yousif, Bhat B, Hani G. Yousif, Guda EN. Therapeutic use of autologous hematopoietic bone marrow transplant combined with concentrated platelet in infected surgical wounds: single-center prospective study [Internet]. Ajbm.net. 2024 [cited 2024 May 02]. Available from: https://ajbm.net/research-article_122620html/

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